GSM6481881: LN-tet-AR, doxycycline, DHT0.1nM, ChIP-AR; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LNCaP-tet-AR
cell line
LNCaP-tet-AR
cell type
Prostate Cancer Cell Line (generated from LNCaP)
treatment
0.25 ug/ml doxycycline 0.1 nM DHT
Sequenced DNA Library
library_name
GSM6481881
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde and then sonicated. DNA were immunoprecipitated with AR antibody and protein A beads. After reverse crosslinking, DNA were purified with Qiagen PCR purification kit. ChIP DNA Library were prepared with NEBNext ChIP-Seq Library Prep Reagent set for Illumina and NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)