Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP-tet-AR
cell line
LNCaP-tet-AR
cell type
Prostate Cancer Cell Line (generated from LNCaP)
treatment
0.25 ug/ml doxycycline 0.1 nM DHT

Sequenced DNA Library

library_name
GSM6481881
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde and then sonicated. DNA were immunoprecipitated with AR antibody and protein A beads. After reverse crosslinking, DNA were purified with Qiagen PCR purification kit. ChIP DNA Library were prepared with NEBNext ChIP-Seq Library Prep Reagent set for Illumina and NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
53682561
Reads aligned (%)
14.9
Duplicates removed (%)
47.2
Number of peaks
1502 (qval < 1E-05)

hg19

Number of total reads
53682561
Reads aligned (%)
14.8
Duplicates removed (%)
47.8
Number of peaks
1471 (qval < 1E-05)

Base call quality data from DBCLS SRA